| 产品编号 | bs-11661R |
| 英文名称 | KCNE3 |
| 中文名称 | 钾离子通道蛋白家族成员3抗体 |
| 别 名 | Cardiac voltage gated potassium channel accessory subunit; HOKPP; KCNE 3; Minimum potassium ion channel related peptide 2; minK related peptide 2; MiRP 2; MiRP2; Potassium voltage gated channel subfamily E member 3; Potassium voltage gated channel, Isk related family, member 3; Voltage gated K+ channel subunit MIRP2; KCNE3_HUMAN. |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞膜受体 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KCNE3: 51-103/103 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to modulate the gating kinetics and enhance stability of the multimeric complex. This gene is prominently expressed in the kidney. A missense mutation in this gene is associated with hypokalemic periodic paralysis. [provided by RefSeq, Jul 2008]. Function: KCNE3 (also known as MiRP2) is an ancillary protein that functions as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. It modulates the gating kinetics of the complex, as well as enhancing channel stability. The complex of KCNE3 with KCNQ1/KCLQT1 is postulated form the intestinal cAMP-stimulated potassium channel that is involved in chloride secretion. KCNE3 in complex with KCNC4/Kv3.4 is thought to form the subthreshold voltage-gated potassium channel that establishes the resting membrane potential in skeletal muscle. Altered activity of the KCNE3/Kv3.4 channel induced by the Abeta peptide is thought to result in the upregulation of the transient outward potassium current, a significant step in the etiology of Alzheimer's disease. Subunit: Associates with KCNC4/Kv3.4. May associate with KCNQ1/KCLQT1. Subcellular Location: Cell Membrane Tissue Specificity: Widely expressed with highest levels in kidney and moderate levels in small intestine. DISEASE: Defects in KCNE3 are the cause of Brugada syndrome type 6 BRGDA6) [MIM:613119]. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Similarity: Belongs to the potassium channel KCNE family. SWISS: Q9Y6H6 Gene ID: 10008 Database links: Entrez Gene: 101144160 Gorilla Entrez Gene: 10008 Human Entrez Gene: 57442 Mouse Omim: 604433 Human SwissProt: Q9Y6H6 Human SwissProt: Q9WTW2 Mouse Unigene: 523899 Human Unigene: 44843 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
