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GLIS2 Rabbit pAb (bs-11566R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-11566R
英文名称 GLIS2 Rabbit pAb
中文名称 GLIS2蛋白抗体
别    名 NPHP7; NKL; GLI kruppel family member 2; GLI similar 2; GLI-similar 2; GLIS 2; GLIS family zinc finger 2; glis2; GLIS2_HUMAN; Kruppel like zinc finger protein GLIS2; Neuronal Krueppel-like protein; Tax helper protein; THP; Zinc finger protein GLI2; Zinc f  
研究领域 神经生物学  信号转导  干细胞  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog)
产品应用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 56 kDa
检测分子量
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GLIS2: 271-350/524 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 GLIS2 is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family. By recruiting the corepressors CtBP1 and HDAC3, GLIS2 represses the transcriptional activation mediated by ∫-catenin in the Wnt pathway. GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation. Mutations of GLIS2 may be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis. GLIS2 contains five tandem Cys(2)-His(2) zinc finger motifs that exhibit the highest homology to those of members of the GLI and Zic subfamilies of Krüppel-like proteins. GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta.

Function:
Can act either as a transcription repressor or as a transcription activator, depending on the cell context. Represses the transcriptional activation mediated by CTNNB1 in the Wnt pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.

Subunit:
Interacts with CTBP1 and HDAC3 (By similarity). Interacts with CTNNB1 (By similarity). Interacts with SUFU (By similarity). Interacts with CTNND1.

Subcellular Location:
Nucleus speckle. Cytoplasm.

Tissue Specificity:
Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.

Post-translational modifications:
C-terminus cleavage is induced by interaction with CTNND1 and enhanced by Src tyrosine kinase

DISEASE:
Defects in GLIS2 are the cause of nephronophthisis type 7 (NPHP7) [MIM:611498]. NPHP7 is an autosomal recessive disorder resulting in end-stage renal disease during childhood or adolescence. It is a progressive tubulo-interstitial kidney disorder histologically characterized by modifications of the tubules with thickening of the basement membrane, interstitial fibrosis and, in the advanced stages, medullary cysts.

Similarity:
Belongs to the GLI C2H2-type zinc-finger protein family.
Contains 5 C2H2-type zinc fingers.

SWISS:
Q9BZE0

Gene ID:
84662

Database links:

Entrez Gene: 374023 Chicken

Entrez Gene: 84662 Human

Entrez Gene: 83396 Mouse

Entrez Gene: 302946 Rat

Omim: 608539 Human

SwissProt: Q9BZE0 Human

SwissProt: Q8R4X9 Mouse

SwissProt: Q8VDL9 Mouse

SwissProt: Q99MY6 Mouse

SwissProt: Q99P73 Mouse

Unigene: 592087 Human

Unigene: 134072 Mouse



产品图片
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLIS2) Polyclonal Antibody, Unconjugated (bs-11566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLIS2) Polyclonal Antibody, Unconjugated (bs-11566R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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