产品编号 | bs-11548R |
英文名称 | Phospho-Dab1 (Tyr198) |
中文名称 | 磷酸化磷脂蛋白受体Dab1抗体 |
别 名 | Dab1 (phospho Y198); Dab1 (Phospho-Tyr198) ; Dab 1; Dab1; Dab-1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari. |
产品类型 | 磷酸化抗体 |
研究领域 | 细胞生物 神经生物学 信号转导 细胞膜受体 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat,Human (predicted: Rabbit,Horse,Cow,Pig,Dog,Chicken,Mouse) |
产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 65kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Dab1 around the phosphorylation site of Tyr198: V(p-Y)QY |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008] Function: Adapter molecule functioning in neural development. May regulate SIAH1 activity. Subunit: Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1. Interacts with LRP1. Post-translational modifications: Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling. Similarity: Contains 1 PID domain. SWISS: O75553 Gene ID: 1600 Database links: Entrez Gene: 1600 Human Entrez Gene: 13131 Mouse Entrez Gene: 374083 Chicken Omim: 603448 Human SwissProt: O75553 Human SwissProt: P97318 Mouse Unigene: 477370 Human Unigene: 289682 Mouse Unigene: 206534 Rat
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产品图片 |
Sample:
Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human SH-SY5Y cell lysates Primary: Anti-Phospho-Dab1 (Tyr198) (bs-11548R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 68 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody, Unconjugated(bs-11548R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |