| 产品编号 | bs-11533R |
| 英文名称 | PCSK2/Proprotein Convertase 2 Rabbit pAb |
| 中文名称 | 神经内分泌转化酶2抗体 |
| 别 名 | KEX2 like endoprotease 2; KEX2-like endoprotease 2; NEC 2; NEC2; NEC2_HUMAN; Neuroendocrine convertase 2; PC2; PCSK2; Prohormone convertase 2; Proprotein convertase 2; Proprotein convertase subtilisin/kexin type 2; SPC2. |
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Specific References (1) | bs-11533R has been referenced in 1 publications.
[IF=2.17] van Rijn, Sarah J., et al. "Expression and clinical relevance of paired box protein 7 and sex determining region Y-box 2 in canine corticotroph pituitary adenomas." The Veterinary Journal (2015). IHC-P ; Dog.
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| 研究领域 | 细胞生物 神经生物学 信号转导 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Sheep,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Proprotein Convertase 2: 201-300/638 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the subtilisin-like proprotein convertase family. These enzymes process latent precursor proteins into their biologically active products. The encoded protein plays a critical role in hormone biosynthesis by processing a variety of prohormones including proinsulin, proopiomelanocortin and proluteinizing-hormone-releasing hormone. Single nucleotide polymorphisms in this gene may increase susceptibility to myocardial infarction and type 2 diabetes. This gene may also play a role in tumor development and progression. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]. Function: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Subcellular Location: Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules. Similarity: Belongs to the peptidase S8 family. Furin subfamily. SWISS: P16519 Gene ID: 5126 Database links: Entrez Gene: 5126 Human Entrez Gene: 18549 Mouse Omim: 162151 Human SwissProt: P16519 Human SwissProt: P21661 Mouse Unigene: 315186 Human Unigene: 294493 Mouse Unigene: 447466 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (PCSK2/Proprotein Convertase 2) Polyclonal Antibody, Unconjugated (bs-11533R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (PCSK2/Proprotein Convertase 2) Polyclonal Antibody, Unconjugated (bs-11533R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
