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Rabbit Anti-phospho-IRE1a (Ser 726)  antibody (bs-4308R)
~~~促销,代码KX240301~~~
~~~促销,代码KX240302~~~
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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
大包装/询价

产品编号 bs-4308R
英文名称 phospho-IRE1a (Ser 726)
中文名称 磷酸化内质网核信号转导蛋白a1抗体
别    名 p-IRE1a (phospho-Ser 726); IRE1 (phospho S726) ; IRE1 alpha (p-Ser726); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring protein 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; Protein kinase/endoribonuclease; Serine/threonine protein kinase/endoribonuclease IRE1.ERN1_HUMAN   
Specific References  (11)     |     bs-4308R has been referenced in 11 publications.
[IF=5.878] Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. 2021 Dec 02  WB ;  Mouse ,Human.  
[IF=5.778] Yuting Wang. et al. Polybrominated diphenyl ethers quinone-induced intracellular protein oxidative damage triggers ubiquitin-proteasome and autophagy-lysosomal system activation in LO2 cells. Chemosphere. 2021 Jul;275:130034  WB ;  Human.  
[IF=5.546] Donghui Yang. et al. Melatonin alleviates LPS‐induced endoplasmic reticulum stress and inflammation in spermatogonial stem cells. J Cell Physiol. 2021 May;236(5):3536-3551  WB,IF,IHC ;  Mouse.  
[IF=5.193] Xu S et al. Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stress. J Neuroinflammation. 2019 Feb 13;16(1):35.  WB ;  Human.  
[IF=4.86] Jiang, Mingfang, et al. "Down-regulation of miR-384-5p Attenuates Rotenone-induced Neurotoxicity in Dopaminergic SH-SY5Y Cells Through Inhibiting Endoplasmic Reticulum Stress." American Journal of Physiology-Cell Physiology (2016): ajpcell-00226.  WB ;  Human.  
[IF=4.19] Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).  WB ;  Human.  
[IF=4.125] Harada,et al.Vesicular nucleotide transporter mediates ATP release and migration in neutrophils.(2018) The Journal of Biological Chemistry. 293:3770-3779.  WB ;  Mouse.  
[IF=3.858] Li,et al.Silver nanoparticles induce SH-SY5Y cell apoptosis via endoplasmic reticulum- and mitochondrial pathways that lengthen endoplasmic reticulum-mitochondria contact sites and alter inositol-3-phosphate receptor function.(2018) Toxicology Letters. 285:156-167.  WB ;  Human.  
[IF=2.776] He Q et al. Titanium dioxide nanoparticles induce mouse hippocampal neuron apoptosis via oxidative stress- and calcium imbalance-mediated endoplasmic reticulum stress. Environ Toxicol Pharmacol. 2018 Oct;63:6-15.  WB ;  Mouse.  
[IF=2.557] Jie Zheng. et al. Icariin attenuates ischaemic stroke through suppressing inflammation mediated by endoplasmic reticulum stress signalling pathway in rats. CLIN EXP PHARMACOL P. 2022 May 02  WB ;  Rat.  
[IF=2.092] Zhang Hao. et al. Protective effects of pterostilbene against hepatic damage, redox imbalance, mitochondrial dysfunction, and endoplasmic reticulum stress in weanling piglets. J Anim Sci. 2020 Oct;98(10):  WB ;  Pig.  
产品类型 磷酸化抗体 
研究领域 细胞生物  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human,Mouse,Rat (predicted: Dog,Pig,Cow,Horse,Rabbit)
产品应用 IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test, ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 107kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human IRE1a around the phosphorylation site of Ser726 : SF(p-S)RR 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Function:
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Subunit:
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Tissue Specificity:
Ubiquitously expressed. High levels observed in pancreatic tissue.

Post-translational modifications:
Autophosphorylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.

SWISS:
O75460

Gene ID:
2081

Database links:

Entrez Gene: 2081 Human

Entrez Gene: 78943 Mouse

Entrez Gene: 498013 Rat

Omim: 604033 Human

SwissProt: O75460 Human

SwissProt: Q9EQY0 Mouse

Unigene: 133982 Human

Unigene: 592041 Human

Unigene: 700027 Human

Unigene: 20452 Mouse

Unigene: 340943 Mouse

Unigene: 226435 Rat



产品图片
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1a (Ser 726)) Polyclonal Antibody, Unconjugated (bs-4308R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-phospho-IRE1a (Ser 726) antibody (bs-4308R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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