| 产品编号 | bs-11294R |
| 英文名称 | Rabbit Anti-HOXA6 antibody |
| 中文名称 | 同源盒基因HOXA6蛋白抗体 |
| 别 名 | HOX1B; Homeo box 1B; Homeo box A6; Homeobox 1B; Homeobox A6; Homeobox protein Hox A6; Homeobox protein Hox-1B; Homeobox protein Hox-A6 antibody Homeobox protein HoxA6; HOX 1; Hox 1B; HOX1; HOX1.2; Hox1B; HOXA6; HX A6; HXA6; HXA6_HUMAN. |
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Specific References (2) | bs-11294R has been referenced in 2 publications.
[IF=5.145] Deng J et al. Oxaliplatin‐induced neuropathic pain involves HOXA6 via a TET1‐dependent demethylation of the SOX10 promoter. Int J Cancer. 2020 Nov 1;147(9):2503-2514. IHC ; Rat.
[IF=3.269] Weifeng Wang. et al. Down-regulated long non-coding RNA LHFPL3 antisense RNA 1 inhibits the radiotherapy resistance of nasopharyngeal carcinoma via modulating microRNA-143-5p/homeobox A6 axis. Bioengineered. 2022;13(3):5421-5433 WB ; Human.
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| 研究领域 | 细胞生物 发育生物学 神经生物学 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Sheep,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23kDa |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HOXA6/HOX1B: 171-233/233 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Hox homeobox genes encode proteins that are transcriptional regulators with an established role in embryonic development. HoxA6 (homeobox A6), also known as HOX1B, is a 233 amino acid protein that localizes to the nucleus. Expressed during embryonic development, HoxA6 functions as a sequence-specific DNA-binding transcription factor that is part of a regulatory mechanism that provides cells with positional identities during development. Via its ability to bind DNA, HoxA6 plays an important role in the regulation of gene expression, as well as morphogenesis and differentiation. The gene encoding HoxA6 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Defects in some of the genes localized to chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Tissue Specificity: Nucleus. Similarity: Belongs to the Antp homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: P31267 Gene ID: 3203 Database links: Entrez Gene: 3203 Human Entrez Gene: 15403 Mouse Omim: 142951 Human SwissProt: P31267 Human SwissProt: P09092 Mouse Unigene: 679517 Human Unigene: 222030 Mouse |
| 产品图片 |
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-HOXA6 (bs-11294R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 26 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (bs-11294R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (bs-11294R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (bs-11294R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (bs-11294R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (bs-11294R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
