| 产品编号 | bs-11257R |
| 英文名称 | RAB10 Rabbit pAb |
| 中文名称 | G蛋白结合蛋白RAB10抗体 |
| 别 名 | GTP binding protein RAB10; Rab 10; Rab-10; RAB10 member RAS oncogene family; RAB10_HUMAN; Ras related GTP binding protein; Ras related GTP binding protein RAB10; Ras-related protein Rab-10. |
| 研究领域 | 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Sheep,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RAB10: 41-140/200 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21 (1). Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport (1,2). The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function (1-6). The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles (9). Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway (3-8). Function: The small GTPases Rab are key regulators ofintracellular membrane trafficking, from the formation of transportvesicles to their fusion with membranes. Rabs cycle between aninactive GDP-bound form and an active GTP-bound form that is ableto recruit to membranes different set of downstream effectorsdirectly responsible for vesicle formation, movement, tethering andfusion (By similarity). That Rab is mainly involved in thebiosynthetic transport of proteins from the Golgi to the plasmamembrane. Regulates, for instance, SLC2A4/GLUT4 glucosetransporter-enriched vesicles delivery to the plasma membrane. Inparallel, it regulates the transport of TLR4, a toll-like receptorto the plasma membrane and therefore may be important for innateimmune response. Plays also a specific role in asymmetric proteintransport to the plasma membrane within the polarized neuron andepithelial cells. In neurons, it is involved in axonogenesisthrough regulation of vesicular membrane trafficking toward theaxonal plasma membrane while in epithelial cells, it regulatestransport from the Golgi to the basolateral membrane. Moreover, mayplay a role in the basolateral recycling pathway and in phagosomematuration. According to PubMed:23263280, may play a role inendoplasmic reticulum dynamics and morphology controllingtubulation along microtubules and tubules fusion. [ENZYME REGULATION] Rab activation is generally mediated by aguanine exchange factor (GEF), while inactivation throughhydrolysis of bound GTP is catalyzed by a GTPase activating protein(GAP) (By similarity). That Rab is activated by the DENND4C guanineexchange factor (GEF). Subunit: Interacts with MYO5A; mediates the transport to theplasma membrane of SLC2A4/GLUT4 storage vesicles. Interacts withGDI1 and maybe with GDI2; negatively regulates RAB10 associationwith membranes and activation. Interacts (GDP-bound form) withLLGL1; the interaction is direct and promotes RAB10 associationwith membranes and activation through competition with the Rabinhibitor GDI1 (By similarity). Interacts with EXOC4; probablyassociates with the exocyst (By similarity). Subcellular Location: Cytoplasmic vesicle membrane; Lipid-anchor(Probable); Cytoplasmic side (Probable). Golgi apparatus,trans-Golgi network membrane (By similarity). Endosome membrane.Recycling endosome membrane. Cytoplasmic vesicle, phagosomemembrane (By similarity). Cell projection, cilium. Endoplasmicreticulum membrane. Note=Associates with SLC2A4/GLUT4 storagevesicles. Localizes to the base of the cilium. Transientlyassociates with phagosomes (By similarity). According toPubMed:23263280 localizes to the endoplasmic reticulum at domainsof new tubule growth. DISEASE: Belongs to the small GTPase superfamily. Rab family. Similarity: Belongs to the small GTPase superfamily. Rab family. Gene ID: 10890 Database links: Entrez Gene: 421994 Chicken Entrez Gene: 19325 Mouse SwissProt: P61027 Mouse Unigene: 378993 Mouse Unigene: 486858 Mouse Unigene: 65864 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB10 Polyclonal Antibody, Unconjugated (bs-11257R) at 1:200 overnight at 4°C, followed by conjuga
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB10 Polyclonal Antibody, Unconjugated (bs-11257R) at 1:200 overnight at 4°C, followed by conjugati
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB10 Polyclonal Antibody, Unconjugated (bs-11257R) at 1:200 overnight at 4°C, followed by conjuga
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
