| 产品编号 | bs-11218R |
| 英文名称 | Pumilio 1 Rabbit pAb |
| 中文名称 | PUM1蛋白抗体 |
| 别 名 | HSPUM; KIAA0099; PUM 1; PUM L1; PUM1; PUM1_HUMAN; PUMH 1; PUMH; PUMH1; Pumilio homolog 1; Pumilio-1; Pumilio1; PUML 1; PUML1. |
| 研究领域 | 发育生物学 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Dog) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 126 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pumilio 1: 201-300/1186 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pumilio 1, also known as PUM1, PUMH1 (Pumilio homolog 1), HSPUM, PUMH or PUML1, is a homolog of the Drosophila Pumilio protein and belongs to the PUF family. The PUF family is comprised of evolutionarily conserved proteins that contain a C-terminal RNA binding domain made up of eight highly conserved tandem repeats. PUF proteins function as sequence-specific RNA-binding proteins and bind NREs (nanos response elements) in the 3’-untranslated regions of target mRNAs. They play an important role mediating mRNA stabilization and repressing translation. Pumilio 1 is a typical PUF protein expressed in fetal tissues as well as adult stomach, kidney, intestine, muscle, brain and heart tissues. Pumilio 1 localizes to the cytoplasm and is believed to participate in cell fate, cell development, cell differentiation and maintenance of somatic stem cells. Function: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. May be required to support proliferation and self-renewal of stem cells (By similarity). Subunit: Binds in a sequence-specific manner to the 3'-UTR of some mRNAs. Subcellular Location: Cytoplasm (Probable). Tissue Specificity: Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested. Similarity: Contains 1 PUM-HD domain. Contains 8 pumilio repeats. SWISS: Q14671 Gene ID: 9698 Database links: Entrez Gene: 9698 Human Entrez Gene: 80912 Mouse Omim: 607204 Human SwissProt: Q14671 Human SwissProt: Q80U78 Mouse Unigene: 281707 Human Unigene: 440206 Mouse |
| 产品图片 |
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- Pumilio 1 (bs-11218R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 126 kD
Observed band size: 126 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
