| 产品编号 | bs-11201R |
| 英文名称 | Musashi 1 Rabbit pAb |
| 中文名称 | 神经干细胞RNA结合蛋白Musashi1抗体 |
| 别 名 | Musashi 1/Msi1; Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1. |
| 研究领域 | 肿瘤 神经生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Musashi 1: 66-150/362 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary. Function: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Subcellular Location: Cytoplasm. Nucleus. Tissue Specificity: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Similarity: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains. SWISS: O43347 Gene ID: 4440 Database links: Entrez Gene: 4440 Human Entrez Gene: 17690 Mouse Omim: 603328 Human SwissProt: O43347 Human SwissProt: Q61474 Mouse Unigene: 158311 Human Unigene: 5077 Mouse Unigene: 162146 Rat Musashi1是一种进化保守的RNA结合蛋白，在维持干细胞状态、分化和肿瘤发生方面起着重要作用。Musashi1 选择性地表达在神经前体细胞上,包括神经干细胞上。在神经系统外，Musashi1还是肠干细胞的选择性标记。这些组织干细胞或未成熟细胞Musashi1的表达，表明Musashi1在转录后基因调节阶段维持这些细胞未分化状态起重要作用。Musashi1过度表达通过依赖RBP2Jk的旁路激活Notch1，而Notch信号途径功能为诱导哺乳动物神经干细胞自我更新。通过musashi1-P-小鼠培养脑细胞的Musashi蛋白产物反义去除研究,发现这些基因在维持神经干细胞未分化状态起着重要的作用。 Musashi抑制m-Numb转录的分子机制尚待进一步研究。Musashi1有可能除转录调控外还参与其他调控途径。另外，Musashi1还表达在一些脑肿瘤的特殊类型(这些肿瘤可能起源自非成熟脑细胞)，并且表达水平和肿瘤的恶性程度及增殖能力相关。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; A
Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal go
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat
SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Musashi 1) polyclonal Antibody, Unconjugated (bs-11201R) 1:100, 90 minutes at 3
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Musashi 1 antibody (bs-11201R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Norma
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
