| 产品编号 | bs-11191R |
| 英文名称 | Rabbit Anti-MRF/C11orf9 antibody |
| 中文名称 | 髓鞘基因调控因子抗体 |
| 别 名 | C11orf9; Chromosome 11 open reading frame 9; Gm1804; gm98; KIAA0954; MGC10781; MRF; MRF_HUMAN; Myelin gene regulatory factor; Ndt80. |
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Specific References (1) | bs-11191R has been referenced in 1 publications.
[IF=3.47] Xiaowei Yu. et al. Nanophthalmos-Associated MYRF Gene Mutation Causes Ciliary Zonule Defects in Mice. Invest Ophth Vis Sci. 2021 Mar;62(3):1-1 WB,IHC ; Mouse.
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| 研究领域 | 细胞生物 免疫学 神经生物学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 124kDa |
| 细胞定位 | 细胞核 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRF: 401-500/1151 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. Function: Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. Subunit: Belongs to the MRF family. Contains 1 NDT80 DNA-binding domain. Subcellular Location: Membrane. Nucleus. Tissue Specificity: Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina. Similarity: Belongs to the MRF family. Contains 1 NDT80 DNA-binding domain. SWISS: Q9Y2G1 Gene ID: 745 Database links: Entrez Gene: 225908 Mouse SwissProt: Q3UR85 Mouse Unigene: 329716 Mouse |
| 产品图片 |
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Sample:
Lane 1: Human A549 cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human U2OS cell lysates
Lane 3: Human A431 cell lysates
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- MRF/C11orf9 (bs-11191R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(bs-11191R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
