| 产品编号 | bs-11167R |
| 英文名称 | VGLUT1/BNP1 |
| 中文名称 | 脑特异性钠依赖无机磷转运蛋白抗体 |
| 别 名 | BNPI; Brain specific Na (+) dependent inorganic phosphate cotransporter; Brain specific Na dependent inorganic phosphate cotransporter; Brain-specific Na(+)-dependent inorganic phosphate cotransporter; Slc17a7; Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7; Solute carrier family 17 member 7; Vesicular glutamate transporter 1; VGLU1_HUMAN; VGluT1. |
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Specific References (4) | bs-11167R has been referenced in 4 publications.
[IF=9.995] Qian Fang. et al. YTHDF1 phase separation triggers the fate transition of spermatogonial stem cells by activating the IκB-NF-κB-CCND1 axis. CELL REP. 2023 Apr 14;42(4):112403 IF ; Mouse.
[IF=2.11] Fujita, Yu, et al. "Expression of MEGF10 in cholinergic and glutamatergic neurons." Neuroscience Letters (2017). IHC ; Mouse.
[IF=1.931] Wan, Dong-Feng. et al. Late Exercise Preconditioning Regulates BNP Increasing to Assist the Cardioprotection via Up-Regulation of NPR-A and Down-Regulation of NPR-C in Rat Myocardium. Int J Pept Res Ther. 2022 Jan;28(1):1-11 WB,IHC ; Rat.
[IF=0] Canhong Wang. et al. Agarwood essential oil inhalation exerts antianxiety and antidepressant effects via the regulation of Glu/GABA system homeostasis. BIOMED REP. 2023 Feb;18(2):1-11 WB ; Mouse.
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| 研究领域 | 神经生物学 信号转导 细胞粘附分子 细胞骨架 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat,Mouse,Human (predicted: Rabbit,Horse,Cow,Pig,Dog,Chicken) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human VGLUT1/BNP1: 301-400/560 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
BNPI;
Brain specific Na (+) dependent inorganic phosphate cotransporter;
Brain specific Na dependent inorganic phosphate cotransporter;
Brain-specific Na(+)-dependent inorganic phosphate cotransporter;
Slc17a7;
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7;
Solute carrier family 17 member 7;
Vesicular glutamate transporter 1;
VGLU1_HUMAN;
VGluT1. Function: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Subcellular Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein (Potential). Cell junction, synapse, synaptosome. Tissue Specificity: Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe. Similarity: Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. SWISS: Q9P2U7 Gene ID: 57030 Database links: Entrez Gene: 57030 Human Entrez Gene: 72961 Mouse Omim: 605208 Human SwissProt: Q9P2U7 Human SwissProt: Q3TXX4 Mouse Unigene: 375616 Human Unigene: 255631 Mouse Unigene: 10267 Rat |
| 产品图片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates Lane 2: Human SH-SY5Y cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-VGLUT1/BNP1 (bs-11167R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kDa Observed band size: 60 kDa 
Tissue/cell: human gliomas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VGLUT1/BNP1 Polyclonal Antibody, Unconjugated(bs-11167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VGLUT1/BNP1 Polyclonal Antibody, Unconjugated(bs-11167R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VGLU1) Polyclonal Antibody, Unconjugated (bs-11167R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
