产品编号 | bs-11164R |
英文名称 | Sidekick 1 |
中文名称 | 细胞粘附分子伴侣蛋白1抗体 |
别 名 | FLJ31425; Protein sidekick-1; sdk1; SDK1_HUMAN; sidekick 1 antibody Sidekick homolog 1, cell adhesion molecule (chicken); sidekick1. |
研究领域 | 细胞生物 发育生物学 神经生物学 细胞粘附分子 细胞膜蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Pig,Horse,Sheep) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 242kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Sidekick 1: 1501-1600/2213 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Cell adhesion molecules influence cell growth, differentiation, embryogenesis, immune response and cancer metastasis by networking information from the extracellular matrix to the cell. Sidekick-1 (SDK1) is a 2,213 amino acid single-pass membrane protein that functions as a cell adhesion molecule by guiding axonal terminals to specific synapses in developing neurons. Existing as three alternatively spliced isoforms, Sidekick-1 is expressed in retinal neurons and contains thirteen fibronectin type-III domains and six Ig-like C2-type (immunoglobulin-like) domains. Sidekick-1 expression is upregulated in glomeruli of patients with HIV-associated nephropathy, where it leads to podocyte dysfunction. The gene encoding Sidekick-1 maps to human chromosome 7p22.2 and murine chromosome 5 G2. Function: Cell adhesion protein that guides axonal terminals to specific synapses in developing neurons. Dysregulation of this protein may play an important role in podocyte dysfunction in HIV-associated nephropathy. Subunit: Contains 13 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. Subcellular Location: Membrane. Tissue Specificity: Up-regulated in glomeruli in HIV-associated nephropathy. In diseased glomeruli, significantly overexpressed and the expression is no longer restricted to mesangial cells but includes podocytes and parietal epithelial cells. Similarity: Contains 13 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q7Z5N4 Gene ID: 221935 Database links: Entrez Gene: 221935 Human Omim: 607216 Human SwissProt: Q7Z5N4 Human Unigene: 653013 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |