| 产品编号 | bs-4891R |
| 英文名称 | Rabbit Anti-CD24 antibody |
| 中文名称 | CD24抗体 |
| 别 名 | CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; Cd24a; Ly-52; CD24_HUMAN. |
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Specific References (1) | bs-4891R has been referenced in 1 publications.
[IF=3.869] Sinkemani A et al. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic ConditionsStem Cells Int.2020 Dec 23;2020:8882549. IF ; Rat.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 信号转导 细胞粘附分子 糖蛋白 细胞类型标志物 骨髓细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8kDa |
| 检测分子量 | 30-45 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse CD24: 27-53/76 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Function: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Subunit : Belongs to the CD24 family. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. DISEASE: Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS) [MIM:126200]. A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. SWISS: P24807 Gene ID: 12484 Database links: Entrez Gene: 100133941 Human Entrez Gene: 12484 Mouse Omim: 600074 Human SwissProt: P25063 Human SwissProt: P24807 Mouse Unigene: 644105 Human Unigene: 721443 Human Unigene: 29742 Mouse Unigene: 6007 Rat 神经干细胞标志物 肿瘤标志物 CD24是一种低分子质量高度糖基化的黏附分子, 主要表达在造血系统. 最近研究表明, 在造血系统肿瘤和一些器官的实体瘤表面可见CD24高表达。近几年很多学者认为：CD24在前列腺癌、非小细胞肺癌、鼻咽癌、原发性肝细胞癌、胆管细胞癌、麦克尔细胞癌、神经源性肿瘤、胰腺癌、胃癌、结肠癌、肾细胞癌、B细胞淋巴瘤、乳腺癌等肿瘤组织中表达增强, 且和肿瘤侵袭转移、复发和预后相关。 |
| 产品图片 |
Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Bone tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Cerebrum tissue lysates
Primary: Anti-CD24 (bs-4891R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 8 kD
Observed band size: 33 kD
Blank control: SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-CD24 antibody (bs-4891R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
