| 产品编号 | bs-11055R |
| 英文名称 | Laminin alpha 4 Rabbit pAb |
| 中文名称 | 层粘蛋白α4抗体 |
| 别 名 | LAMA3; LAMA4 1; LAMA4; LAMA4_HUMAN; Laminin alpha 4; Laminin alpha 4 chain; Laminin subunit alpha 4 precursor; Laminin subunit alpha-4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha; Lamininα4; Laminin α4; Laminin-α4; Laminin α 4. |
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Specific References (1) | bs-11055R has been referenced in 1 publications.
[IF=2.795] Wan Q et al. Overexpression of laminin α4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling. Connect Tissue Res. 2020 Jun 10;1-11. IHC-P ; Rabbit.
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| 研究领域 | 肿瘤 神经生物学 信号转导 细胞粘附分子 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 产品应用 | ICC/IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 200 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Laminin alpha 4: 901-1000/1823 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Laminins are essential and abundant structural non-collagenous glyco- proteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing Type IV collagen networks, Nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of a, b, and g chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human Laminin a-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta. Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Subunit: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-4 is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423). Subcellular Location: Secreted > extracellular space > extracellular matrix > basement membrane. Major component. Tissue Specificity: In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis. Similarity: Contains 4 laminin EGF-like domains. Contains 5 laminin G-like domains. SWISS: Q16363 Gene ID: 3910 Database links: Entrez Gene: 3910 Human Entrez Gene: 16775 Mouse SwissProt: Q16363 Human SwissProt: P97927 Mouse
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| 产品图片 |
Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin al
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
