| 产品编号 | bs-11014R |
| 英文名称 | ILVBL Rabbit pAb |
| 中文名称 | 乙酰乳酸合成酶蛋白抗体 |
| 别 名 | Acetolactate synthase homolog; Acetolactate synthase like protein; Acetolactate synthase-like protein; AHAS; ILV2H; 209L8; IlvB(bacterial acetolactate synthase) like; IlvB like protein; IlvB-like protein; Ilvbl; ILVBL_HUMAN. |
| 研究领域 | 心血管 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Pig,Dog,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ILVBL: 301-400/632 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ILVBL is a 632 amino acid single-pass membrane protein that belongs to the TPP enzyme family. Expressed in the majority of tissues, ILVBL has the highest level of expression in heart, pancreas and placenta. ILVBL is highly homologous to several bacterial enzymes, including the B isozyme of the large catalytic subunit of E. coli acetohydroxy-acid synthase (AHAS) and the oxalyl-coA decarboxylase of O. formigenes, that utilize thiamine pyrophosphate as a cofactor. ILVBL binds one magnesium ion and one thiamine pyrophosphate per subunit, and may catalyze the initial step in branched-chain amino acid biosynthesis. The gene encoding ILVBL maps to human chromosome 19p13.12 and mouse chromosome 10 C1. Subcellular Location: Membrane. Tissue Specificity: Expressed in all tissues tested, with highest expression in heart, pancreas and placenta. Similarity: Belongs to the TPP enzyme family. SWISS: A1L0T0 Gene ID: 10994 Database links: Entrez Gene: 10994 Human Omim: 605770 Human SwissProt: A1L0T0 Human Unigene: 78880 Human |
| 产品图片 |
Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-ILVBL (bs-11014R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD
Sample:
Hcclm3(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti- ILVBL (bs-11014R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-ILVBL antibody (bs-11014R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
