| 产品编号 | bs-11013R |
| 英文名称 | ILDR1 Rabbit pAb |
| 中文名称 | 免疫球蛋白样结构域受体1抗体 |
| 别 名 | DFNB42; ILDR1alpha; ILDR1alpha'; ILDR1beta; ILDR1_HUMAN; ILDR1; Angulin-2; ILDR1_MOUSE; |
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Specific References (1) | bs-11013R has been referenced in 1 publications.
[IF=6.6] Franziska Weiß. et al. MarvelD3 Is Upregulated in Ulcerative Colitis and Has Attenuating Effects during Colitis Indirectly Stabilizing the Intestinal Barrier. CELLS-BASEL. 2022 Jan;11(9):1541 WB ; Human.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ILDR1: 101-200/546 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ILDR1 is a putative membrane receptor. It belongs to the immunoglobulin superfamily, LISCH7 family. It is mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver. Function: Putative membrane receptor. Subunit: Homooligomer. Subcellular Location: Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol). Tissue Specificity: Mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver. DISEASE: Defects in ILDR1 are the cause of deafness autosomal recessive type 42 (DFNB42) [MIM:609646]; also called non-syndromic sensorineural deafness autosomal recessive type 42. DFNB42 is a prelingual, non-progressive form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Similarity: Belongs to the immunoglobulin superfamily. LISCH7 family. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q86SU0 Gene ID: 286676 Database links: Entrez Gene: 286676 Human Entrez Gene: 106347 Mouse Omim: 609739 Human SwissProt: Q86SU0 Human SwissProt: Q8CBR1 Mouse Unigene: 98484 Human Unigene: 17807 Mouse |
| 产品图片 |
Sample:
Prostate(Rat) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56 kD
Sample:
Pancreas(Mouse) Cell Lysate at 40 ug
Primary: Anti-ILDR1 (bs-11013R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 56kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (bs-11013R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
