| 产品编号 | bs-9971R |
| 英文名称 | CREPT Rabbit pAb |
| 中文名称 | 肿瘤高表达细胞周期相关蛋白抗体 |
| 别 名 | C20orf77; Cell cycle related and expression elevated protein in tumor; Chromosome 20 open reading frame 77; CREPT; dJ1057B20.2; DKFZp434P0735; FLJ44520; Hypothetical protein LOC58490; Uncharacterized protein C20orf77; RPR1B_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Chicken) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CREPT: 221-320/326 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Interacts with phosphorylated C-terminal heptapeptide
repeat domain (CTD) of the largest RNA polymerase II subunit
POLR2A, and participates in dephosphorylation of the CTD.
Transcriptional regulator which enhances expression of CCND1.
Promotes binding of RNA polymerase II to the CCDN1 promoter and to
the termination region before the poly-A site but decreases its
binding after the poly-A site. Prevents RNA polymerase II from
reading through the 3' end termination site and may allow it to be
recruited back to the promoter through promotion of the formation
of a chromatin loop. Also enhances the transcription of a number of
other cell cycle-related genes including CDK2, CDK4, CDK6 and
cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Function: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. Subunit: Associates with the RNA polymerase II complex. Subcellular Location: Nucleus. Tissue Specificity: Preferentially expressed in a range of tumor tissues including colon, lung, liver, breast, prostate, stomach, uterine endometrium and cervical cancers with higher levels in tumors than in adjacent non-tumor tissue (at protein level). Similarity: Belongs to the UPF0400 (RTT103) family. Contains 1 CID domain. SWISS: Q9NQG5 Gene ID: 58490 Database links: Entrez Gene: 58490 Human Entrez Gene: 70470 Mouse Omim: 614694 Human SwissProt: Q9NQG5 Human SwissProt: Q9CSU0 Mouse Unigene: 278839 Human |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- CREPT (bs-9971R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
