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Myocardin Rabbit pAb (bs-9472R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-9472R
英文名称 Myocardin Rabbit pAb
中文名称 促血管平滑肌细胞分化因子抗体
别    名 MYCD; MYCD_HUMAN; Myocardin; Myocd.  
Specific References  (2)     |     bs-9472R has been referenced in 2 publications.
[IF=17.521] Hao Sun. et al. Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner. Advanced Science. 2023 Apr;:2207020  IF ;  Human.  
[IF=0.8] Morita et al. Mechanisms underlying a decrease in KCl-induced contraction after long-term serum-free organ culture of rat isolated mesenteric artery. (2014) J.Vet.Med.Sci. 76:963-9  WB ;  Rat.  
研究领域 心血管  免疫学  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse)
产品应用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 103 kDa
检测分子量
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Myocardin: 456-560/461-560/938 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.

Function:
Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis).

Subunit:
Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation. Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

Post-translational modifications:
Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.

Similarity:
Contains 3 RPEL repeats.
Contains 1 SAP domain.

SWISS:
Q8IZQ8

Gene ID:
93649

Database links:

Entrez Gene: 93649 Human

Entrez Gene: 214384 Mouse

Entrez Gene: 396571 Pig

Entrez Gene: 246297 Rat

Omim: 606127 Human

SwissProt: Q8IZQ8 Human

SwissProt: Q8VIM5 Mouse

SwissProt: Q8R5I7 Rat

Unigene: 567641 Human

Unigene: 32257 Mouse

Unigene: 163103 Rat



产品图片
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Myocardin Polyclonal Antibody, Unconjugated(bs-9472R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Myocardin antibody (bs-9472R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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