| 产品编号 | bs-9897R |
| 英文名称 | Glycoprotein VI Rabbit pAb |
| 中文名称 | 糖蛋白VI/六糖蛋白抗体 |
| 别 名 | BDPLT11; GPIV; GPVI; GPVI_HUMAN; GP6; Glycoprotein 6; |
| 研究领域 | 心血管 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Cow,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 35 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPVI/Glycoprotein VI: 121-220/339 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]. Function: Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma-chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. Subunit: Associated with Fc receptor gamma chain. The GPVI-FcRgamma complex is associated with the Src kinase family Fyn and Lyn. Subcellular Location: Isoform 1: Cell membrane; Single-pass membrane protein. Isoform 2: Cell membrane; Single-pass membrane protein. Tissue Specificity: Megakaryocytes and platelets. Post-translational modifications: N-linked glycosylation at Asn-92 is not required for the cell surface expression, but contributes to maximal adhesion to type I collagen, collagen-related peptide (CRP), and, to a lesser extent, to the snake venom C-type lectin convulxin (CVX). DISEASE: Defects in GP6 are the cause of bleeding disorder platelet-type 11 (BDPLT11) [MIM:614201]. BDPLT11 is a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. SWISS: Q9HCN6 Gene ID: 51206 Database links: Entrez Gene: 51206 Human
SwissProt: Q9HCN6 Human |
| 产品图片 |
Sample:
Lane 1: Mouse Spleen Lysates
Lane 2: Mouse Testis Lysates
Lane 3: Rat Spleen Lysates
Lane 4: Rat Testis Lysates
Lane 5: Human K562 cell Lysates
Lane 6: Human U-2 OS cell Lysates
Primary: Anti-Glycoprotein VI (bs-9897R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35kDa
Observed band size: 65kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
