| 产品编号 | bs-9877R |
| 英文名称 | Staufen |
| 中文名称 | 双链RNA结合蛋白Staufen抗体 |
| 别 名 | Double stranded RNA binding protein Staufen homolog 1; Double stranded RNA binding protein Staufen homolog; Double-stranded RNA-binding protein Staufen homolog 1; Double-stranded RNA-binding protein Staufen homolog; FLJ25010; MGC124588; STAU; STAU1; STAU1_HUMAN; staufen; Staufen RNA binding protein (Drosophila); Staufen RNA binding protein homolog 1. |
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Specific References (2) | bs-9877R has been referenced in 2 publications.
[IF=9.6] de Morrée, Antoine, et al. "Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence." Proceedings of the National Academy of Sciences 114.43 (2017): E8996-E9005. IF(ICC) ; Mouse.
[IF=9.504] Morree et al. Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence. (2017) Proc.Natl.Acad.Sci.U.S.A. 114:E8996-E9005 IF ; Human, Mouse, Rat, Chicken, Cow, Horse,.
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| 研究领域 | 免疫学 神经生物学 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Chicken,Cow,Horse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63, 126kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Staufen/STAU1: 421-520/577 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long. Function: Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. Subunit: Binds tubulin. Binds with low affinity single-stranded RNA or DNA homopolymers. Interacts with CASC3 in an RNA-dependent manner (By similarity). Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with the influenza virus nonstructural protein NS1. Subcellular Location: Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic. Tissue Specificity: Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta. Similarity: Contains 3 DRBM (double-stranded RNA-binding) domains. SWISS: O95793 Gene ID: 6780 Database links: Entrez Gene: 6780 Human Entrez Gene: 20853 Mouse Omim: 601716 Human SwissProt: O95793 Human SwissProt: Q9Z108 Mouse Unigene: 596704 Human Unigene: 73276 Mouse Unigene: 9028 Rat |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- Staufen (bs-9877R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63/126 kD Observed band size: 55 kD 
Sample:
SW480(Human) Cell Lysate at 30 ug Primary: Anti- Staufen (bs-9877R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63/126 kD Observed band size: 55 kD 
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Staufen) Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Staufen) Polyclonal Antibody, Unconjugated (bs-9877R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
