| 产品编号 | bs-9871R |
| 英文名称 | Sorcin |
| 中文名称 | 抗药蛋白抗体 |
| 别 名 | 22 kDa protein; Calcium binding protein amplified in mutlidrug resistant cells; CP 22; CP-22; CP22; SCN; Sorcin (class 4 gene); Sorcin; SORCN_HUMAN; SRI; V19. |
| 研究领域 | 心血管 结合蛋白 细胞骨架 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 22kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SR1/Sorcin: 131-198/198 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sorcin is a highly conserved protein, with 95% homology between hamster and human sorcin sequences. Sorcin has four putative Ca-binding domains, two of which exhibit strong homology to calmodulin “EF hand” motifs. Calcium binding directly to sorcin has been demonstrated by in vitro assays. Sorcin is closely related to members of calpain and sarcoplasmic Ca2+-binding protein subfamilies. Sorcin undergoes calcium-dependent translocation from the cytosol to cellular membranes. Sorcin binds to and modulates ryanodine receptors and is widely distributed including heart and brain tissues. At the subcellular level, sorcin localizes to T-tubule junctions of cardiac sarcoplasmic reticulum. Function: Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels. Subunit: Homodimer. Interacts with GCA, RYR2 and ANXA7. Subcellular Location: Cytoplasm. Sarcoplasmic reticulum membrane. Relocates to the sarcoplasmic reticulum membrane in response to elevated calcium levels. Tissue Specificity: Detected in cardiac myocytes. Similarity: Contains 4 EF-hand domains. SWISS: P30626 Gene ID: 6717 Database links: Entrez Gene: 6717 Human Entrez Gene: 109552 Mouse Omim: 182520 Human SwissProt: P30626 Human SwissProt: Q6P069 Mouse Unigene: 489040 Human Unigene: 96211 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
