| 产品编号 | bs-4826R |
| 英文名称 | Rabbit Anti-CD83 antibody |
| 中文名称 | CD83抗体 |
| 别 名 | B cell activation 45kDa cell surface glycoprotein Ig superfamily; B cell activation protein; B-cell activation protein; BL11; BL11 PEN; CD83; CD83 antigen(activated B lymphocytes, immunoglobulin superfamily); CD83 antigen; CD83 molecule; CD83_HUMAN; Cell surface glycoprotein; Cell surface protein HB15; HB15; hCD83. |
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Specific References (3) | bs-4826R has been referenced in 3 publications.
[IF=6.203] Huang J et al. E6-regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. International Journal of Oncology 56, no. 4 (2020): 921-931. IHC-P ; Human.
[IF=2.485] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31 IHC ; Mouse.
[IF=2.029] Jian Zheng. et al. Higher CD1a Levels Correlate with PD-L1 Expression and Predict Worse Overall Survival in Triple-Negative Breast Carcinoma. Breast Care. ;:1-9 IHC ; Human.
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| 研究领域 | 淋巴细胞 t-淋巴细胞 b-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat) |
| 产品应用 | Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD83: 32-100/205 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The CD83 antigen is a 186 amino acid single chain glycoprotein. This molecule is a member of the immunoglobulin superfamily and is composed of an extracellular V type immunoglobulin-like single domain, a transmembrane region, and a short, 40 amino acid cytoplasmic tail. CD83 antigen undergoes extensive post translational glycosylation, as the determined Mr is twice the predicted size of the core protein. However, CD83+ cells have a unique cell surface immunophenotype that does not correlate with that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage. CD83+ cells coexpress the highest levels of MHC class II molecules, when compared with other leucocyte lineages. They also coexpress T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell markers (CD13, CD33, CD36) and cytokine receptors, as well as other cell surface molecules. Function: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. Subunit: Monomer. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q01151 Gene ID: 9308 Database links: Entrez Gene: 9308 Human Entrez Gene: 12522 Mouse Omim: 604534 Human SwissProt: Q01151 Human SwissProt: O88324 Mouse Unigene: 595133 Human Unigene: 57175 Mouse Unigene: 20096 Rat |
| 产品图片 |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CD83 antibody (bs-4826R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
