| 产品编号 | bs-9727R |
| 英文名称 | BIN2 Rabbit pAb |
| 中文名称 | 乳腺癌相关蛋白1抗体 |
| 别 名 | BRAP-1; BIN2_HUMAN; BIN2; Breast cancer-associated protein 1; BRAP1; |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BIN2/BRAP1: 21-120/565 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
BAR proteins are characterized by a common N-terminal BAR (bin, amphiphysin and Rvs161/167) domain and are recognized as adaptor proteins that are involved in many cellular processes. BIN1 and BIN2 are BAR proteins that share 61% sequence similarity. BIN1 (Bridging integrator 1) is a ubiquitously expressed regulatory protein for synaptic vesicle endocytosis. BIN1 also interacts with the transcription factors c-Myc and MyoD, potentially functioning as a tumor suppressor. BIN2, also known as Breast cancer-associated protein 1, is a 565 amino acid protein that interacts with BIN1. In contrast to BIN1, BIN2 lacks tumor suppressor features as well as a c-Myc interacting region. BIN2 shows preferred expression in tissues of hematopoietic origin, with high levels found in spleen, thymus, colon, placenta, lymphoid and granulocytic cells. There are two isoforms of BIN2 that are produced as a result of alternative splicing events. Subunit: Interacts with BIN1. Subcellular Location: Cytoplasm. Tissue Specificity: Highest level expression seen in spleen and peripheral blood leukocytes and is also expressed at high levels in thymus, colon and placenta, suggesting preferential expression in hematopoietic tissues. Similarity: Contains 1 BAR domain. SWISS: Q9UBW5 Gene ID: 51411 Database links: Entrez Gene: 51411 Human Omim: 605936 Human SwissProt: Q9UBW5 Human Unigene: 14770 Human |
| 产品图片 |
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-BIN2 (bs-9727R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BIN2) Polyclonal Antibody, Unconjugated (bs-9727R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
