| 产品编号 | bs-8507R |
| 英文名称 | Hairless Rabbit pAb |
| 中文名称 | 无毛发蛋白抗体 |
| 别 名 | HR; ALUNC; AU; HAIR_HUMAN; Hairless protein; Host range; HSA277165; Protein hairless. |
| 研究领域 | 染色质和核信号 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 127 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hairless: 345-460/1189 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Hairless is a 1,189 amino acid protein which is expressed as two isoforms produced by alternative splicing. The two isoforms are expressed in a variety of tissues in varying concentrations. Isoform 1 is more abundant than isoform 2 and is expressed at low levels in kidneys and testis, while isoform 2 is expressed abundantly in skin. Both isoforms are also present together in many tissues and are expressed strongly in small intestine and brain and weakly in trachea. HR is thought to be a transcription factor involved in hair growth. Hair growth occurs in three phases known as anagen, catagen and telogen, which are phases where growth, regression and rest, respectively, are taking place. By unknown mechanisms, HR is thought to regulate one of the hair growth phases and to work with vitamin D receptor (VDR) to regulate hair follicle cycling. Defects in HR may cause two serious ailments, known as alopecia universalis congenita (ALUNC) and atrichia with papular lesions (APL), which is also referred to as congenital atrichia. Both are autosomally recessive impairments. ALUNC is a rare condition in which hair follicles are produced without hair, while APL is a serious disease in which papillary lesions may cover the body and little to no hair is grown. Function: May act as a transcription factor that could act on to regulate one of the phases of hair growth. Subcellular Location: Nucleus. Tissue Specificity: Strongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin. DISEASE: Defects in HR are the cause of alopecia universalis congenita (ALUNC) [MIM:203655]. ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair. Defects in HR are the cause of atrichia with popular lesions (APL) [MIM:209500]; also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair. Defects in HR are the cause of hypotrichosis type 4 (HYPT4) [MIM:146550]. An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood. Similarity: Contains 1 JmjC domain. SWISS: O43593 Gene ID: 55806 Database links: Entrez Gene: 55806 Human Omim: 602302 Human SwissProt: O43593 Human Unigene: 272367 Human Unigene: 41543 Rat |
| 产品图片 |
Sample:
Siha(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- Hairless (bs-8507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 127 kD
Observed band size: 127 kD
Sample:
Hela (Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Hairless (bs-8507R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 127 kD
Observed band size: 120 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
