| 产品编号 | bs-9341R |
| 英文名称 | Rabbit Anti-MARCH7 antibody |
| 中文名称 | 膜相关环指蛋白7抗体 |
| 别 名 | AXO; AXOT; Axotrophin; E3 ubiquitin-protein ligase MARCH7; MARCH VII; MARCH-VII; March7; MARH7_HUMAN; Membrane associated RING CH protein VII; Membrane associated ring finger (C3HC4) 7 E3 ubiquitin protein ligase; membrane associated ring finger C3HC4 7; Membrane associated RING finger protein 7; Membrane-associated RING finger protein 7; Membrane-associated RING-CH protein VII; RING finger protein 177; RNF177. |
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Specific References (5) | bs-9341R has been referenced in 5 publications.
[IF=5.008] Hu, Jianguo, et al. "Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth." Oncotarget 6.14 (2015): 12174. IHC-P ; Human.
[IF=4.743] Xu M et al. Acetate attenuates inflammasome activation through GPR43-mediated Ca 2+-dependent NLRP3 ubiquitination. Exp Mol Med. 2019 Jul 23;51(7):83. WB&IP ; Mouse.
[IF=2.224] Liu L et al. miR-27b-3p/MARCH7 regulates invasion and metastasis of endometrial cancer cells through Snail-mediated pathway. Acta Biochim Biophys Sin (Shanghai). 2019 Apr 22. pii: gmz030. IHC ; Human.
[IF=1.664] Hu et al. Ubiquitin E3 Ligase MARCH7 promotes proliferation and invasion of cervical cancer cells through VAV2-RAC1-CDC42 pathway. (2018) Oncol.Lett. 16:2312-2318 IHC ; Mice.
[IF=1.29] Zhang, Li, et al. "Expression of USP7 and MARCH7 Is Correlated with Poor Prognosis in Epithelial Ovarian Cancer." The Tohoku Journal of Experimental Medicine 239.3 (2016): 165-175. IHC-P ; Human.
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| 研究领域 | 细胞生物 神经生物学 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 78kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MARCH7/Axotrophin: 31-130/704 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Axotrophin is a stem cell gene that encodes a protein which is involved in T lymphocyte regulation (especially in regulating the proliferation) and leukemia inhibitory factor (LIF) release. LIF is a neuropoietic cytokine that is important for stem cell regulation and thymocyte stimulation. Both Axotrophin and LIF are linked to transplantation intolerance. Axotrophin is also involved in corpus callosum differentiation and may play a role in glial cell line-derived neurotrophic factor (GDNF)-dependent sensory neuron survival in the substantia gelatinosa of the adult spinal cord. Axotrophin is primarily expressed in the hippocampus, cortex, purkinje and granule cells of the cerebellum. Function: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. Similarity: Contains 1 RING-CH-type zinc finger. SWISS: Q9H992 Gene ID: 64844 Database links: Entrez Gene: 64844 Human Entrez Gene: 57438 Mouse Omim: 613334 Human SwissProt: Q9H992 Human SwissProt: Q9WV66 Mouse SwissProt: Q5R9W2 Orangutan Unigene: 529272 Human Unigene: 260635 Mouse Unigene: 117620 Rat |
| 产品图片 |
Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-MARCH7 (bs-9341R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78/74 kD
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (MARCH7) Polyclonal Antibody, Unconjugated (bs-9341R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
