| 产品编号 | bs-9325R |
| 英文名称 | ASZ1 Rabbit pAb |
| 中文名称 | 锚定蛋白样蛋白1抗体 |
| 别 名 | Ankyrin repeat, SAM and basic leucine zipper domain containing protein 1; Ankyrin repeat, SAM and basic leucine zipper domain containing protein 1; ALP1; ANKL1; Ankyrin like protein 1; ASZ 1; ASZ1_HUMAN. |
| 研究领域 | 肿瘤 心血管 细胞生物 神经生物学 信号转导 细胞周期蛋白 激酶和磷酸酶 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ASZ1: 151-250/475 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ASZ1 (ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1), also known as ANKL1, ALP1 (Ankyrin-like protein 1), GASZ (germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein) or Orf3, is a 475 amino acid protein that is suggested to function as a signal transducer during germ cell maturation. Localized to the cytoplasm of pachytene spermatocytes, early spermatids and oocytes, ASZ1 is primarily found in adult testis and ovary where it mediates protein-protein interactions. ASZ1 contains six ANK repeats, one SAM (sterile alpha motif) domain and is encoded by a gene located on human chromosome 7q31, a region that may have an important role in the development certain types of human cancer. Function: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation (By similarity). Subunit: Interacts with DDX4, PIWIL1, RANBP9 and TDRD1 (By similarity). Subcellular Location: Cytoplasm (By similarity). Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to pi-bodies, a subset of the nuage which contains primary piRNAs (By similarity). Tissue Specificity: Expressed exclusively in the testis and ovary and at higher levels in the adult testis compared with the adult ovary. Similarity: Contains 6 ANK repeats. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q8WWH4 Gene ID: 136991 Database links: Entrez Gene: 136991 Human SwissProt: Q8WWH4 Human |
| 产品图片 |
Sample:
Ovary(Mouse) Lysate at 40 ug
Spinal cord(Mouse) Lysate at 40 ug
Primary: Anti-ASZ1 (bs-9325R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
