产品编号 | bs-9294R |
英文名称 | Rabbit Anti-BTBD1 antibody |
中文名称 | BTB/POZ结构域蛋白1抗体 |
别 名 | BTB (POZ) domain containing 1; BTB domain containing 1; BTB/POZ domain containing protein 1; BTBD 1; BTBD1; C15orf1; HCV NS5A transactivated protein 8; Hepatitis C virus NS5A transactivated protein 8; NS5ATP8; BTBD1_HUMAN. |
研究领域 | 细胞生物 细胞周期蛋白 转录调节因子 细菌及病毒 细胞分化 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 53kDa |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BTBD1: 101-200/482 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
BTBD1 contains a proline-rich region and a BTB/POZ domain which are typically involved in protein-protein interactions. The C-terminus of BTBD1 binds topoisomerase I and BTBD1 also interacts with TRIM5 isoform Delta. BTBD1 localizes to cytoplasmic bodies and is essential for myogenic differentiation. Function: Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Subunit: Interacts with topoisomerase 1 and with TRIM5 isoform Delta. Interacts with CUL3. Subcellular Location: Cytoplasm, P-body. Cytoplasmic bodies. Tissue Specificity: Ubiquitous; higher levels in heart and skeletal muscle. Similarity: Contains 1 BTB (POZ) domain. SWISS: Q9BSF8 Gene ID: 84280 Database links: Entrez Gene: 84280 Human Entrez Gene: 68815 Mouse SwissProt: Q9BSF8 Human SwissProt: Q80X66 Mouse |
产品图片 |
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (bs-9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (bs-9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD1) Polyclonal Antibody, Unconjugated (bs-9294R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |