| 产品编号 | bs-9257R |
| 英文名称 | RNF150 Rabbit pAb |
| 中文名称 | 环指蛋白150抗体 |
| 别 名 | A630007N06Rik; C030044C12Rik; Greul5; mKIAA1214; RGD1304572; RN150_HUMAN; RNF150; KIAA1214; RN150_MOUSE; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Sheep,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF150: 51-150/347 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in ubiquitin-dependent protein catabolic process. Predicted to be integral component of membrane. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022] Subcellular Location: Contains 1 PA (protease associated) domain. Contains 1 RING-type zinc finger. Similarity: Contains 1 PA (protease associated) domain. Contains 1 RING-type zinc finger. SWISS: Q9ULK6 Gene ID: 57484 Database links: Entrez Gene: 57484 Human Entrez Gene: 330812 Mouse SwissProt: Q9ULK6 Human SwissProt: Q5DTZ6 Mouse Unigene: 659104 Human |
| 产品图片 |
Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti-RNF150 (bs-9257R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-RNF150 (bs-9257R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-RNF150 (bs-9257R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 47 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
