| 产品编号 | bs-9254R |
| 英文名称 | RNF138 Rabbit pAb |
| 中文名称 | 环指蛋白138抗体 |
| 别 名 | E3 ubiquitin protein ligase RNF138; E3 ubiquitin-protein ligase RNF138; hNARF; HSD 4; HSD4; MGC8758; NARF; Nemo like kinase associated RING finger protein; Nemo-like kinase-associated RING finger protein; NLK associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; RN138_HUMAN; Rnf138; STRIN. |
| 研究领域 | 细胞生物 细胞周期蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF138: 51-150/245 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway. Function: E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway. Subunit: Interacts with NLK. Post-translational modifications: Auto-ubiquitinated. Similarity: Contains 1 RING-type zinc finger. SWISS: Q8WVD3 Gene ID: 51444 Database links: Entrez Gene: 51444 Human Entrez Gene: 56515 Mouse SwissProt: Q8WVD3 Human SwissProt: Q9CQE0 Mouse Unigene: 302408 Human Unigene: 253542 Mouse Unigene: 6814 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF138) Polyclonal Antibody, Unconjugated (bs-9254R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
