| 产品编号 | bs-9163R |
| 英文名称 | RNF17 Rabbit pAb |
| 中文名称 | 环指蛋白17抗体 |
| 别 名 | Mmip 2; Mmip2; Ring finger protein 17; RNF 17; SPATA 23; SPATA23; Spermatogenesis associated 23; TDRD 4; TDRD4; Tudor domain containing 4. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 185 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RNF17: 1031-1200/1623 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF17 (ring finger protein 17) or tudor domain-containing protein 4, TDRD4, SPATA23, Mmip-2 or FLJ11045, is a testis-specific protein and and novel key regulator of spermiogenesis containing 1,623 amino acids. By distributing Mad proteins to the cytoplasm, RNF17 regulates the transcriptional activity of c-Myc. Although showing localization in the nucleus, RNF17 is predominantly observed in cytoplasm and is a component of a novel nuage found in male germ cells. The gene encoding RNF17 maps to human chromosome 13q12.12 and encodes one RING-type zinc finger and four tudor domains. As a result of alternative splice events, five RNF17 isoforms exisit. Function: Seems to be involved in regulation of transcriptional activity of MYC. In vitro, inhibits DNA-binding activity of Mad-MAX heterodimers. Can recruit Mad transcriptional repressors (MXD1, MXD3, MXD4 and MXI1) to the cytoplasm. May be involved in spermiogenesis (By similarity). Subunit: Interacts with MXD1, MXD3, MXD4, MXI1 and PIWIL1. Self-associates (By similarity). Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Note=Predominantly found in the cytoplasm. Component of a nuage in male germ cells (an electron-dense spherical cytoplasmic body present in late pachytene and diplotene spermatocytes and in elonging spermatids) (By similarity). Tissue Specificity: Testis specific. Similarity: Contains 1 RING-type zinc finger. Contains 4 Tudor domains. SWISS: Q9BXT8 Gene ID: 56163 Database links: Entrez Gene: 56163 Human Omim: 605793 Human SwissProt: Q9BXT8 Human Unigene: 97464 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF17) Polyclonal Antibody, Unconjugated (bs-9163R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
