| 产品编号 | bs-9097R |
| 英文名称 | Rabbit Anti-ANKS1B antibody |
| 中文名称 | β淀粉样蛋白胞内结构域相关蛋白1抗体 |
| 别 名 | AIDA 1; AIDA-1; Amyloid-beta protein intracellular domain-associated protein 1; Anks1b; Ankyrin repeat and sterile alpha motif domain-containing protein 1B; ANS1B_HUMAN; E2A-PBX1-associated protein; EB-1. |
| 研究领域 | 细胞生物 免疫学 神经生物学 Alzheimer's |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Sheep,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 138 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 细胞外基质 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ANKS1B/AIDA1: 851-1000/1248 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The β-Amyloid protein precursor (AbPP) is a widely expressed transmembrane protein that is processed into the b-Amyloid (Ab) peptide, which accumulates in insoluble plaques in the brain of Alzheimer’s disease patients and AbPP intracellular domain (AID). AID may function as a pro-apoptotic peptide, a regulator of calcium homeostasis and a molecule involved in transcriptional regulation. The AID associated protein 1 (AIDA-1) is highly expressed in the brain and is regulated by AbPP. It interacts with AbPP to play a role in brain development. AIDA-1 also interacts with coilin in Cajal bodies to regulate pre-mRNA splicing. Function: Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells. Isoform 3 can regulate global protein synthesis by altering nucleolar numbers. Isoform 4 may play a role as a modulator of APP processing. Overexpression can down-regulate APP processing. Subunit: Isoform 3 interacts with DLG4. Interacts with EPHA8. Isoform 2 interacts with COIL. Isoform 4 interacts with APP and EPHA8. Isoform 6 interacts with EPHA8. Subcellular Location: Cytoplasm; Nucleus; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendritic spine. Nucleus. Nucleus, Cajal body. The synaptic localization requires DLG4 interaction. Translocation to the nucleus in response to stimulation of NMDA receptors (NMDARs) in a calcium-independent manner and Nucleus. The interaction with APP causes its partial exclusion from the nucleus, when APP is overexpressed. Tissue Specificity: Highly expressed in marrow from patients with pre-B ALL associated with the t(1;19) translocation. Strongly expressed in brain and testis. Expressed in fetal brain. Isoform 4 is highly expressed in brain (at protein level). Isoform 6 is expressed in brain and several cancer cell lines. Post-translational modifications: Isoform 3 nuclear translocation requires an NMDAR-dependent proteolytic cleavage (By similarity). Similarity: Contains 7 ANK repeats. Contains 1 PID domain. Contains 2 SAM (sterile alpha motif) domains. SWISS: Q7Z6G8 Gene ID: 56899 Database links: Entrez Gene: 56899 Human Omim: 607815 Human SwissProt: Q7Z6G8 Human Unigene: 506458 Human |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
