| 产品编号 | bs-9511R |
| 英文名称 | PAR4 Rabbit pAb |
| 中文名称 | 蛋白酶激活受体4抗体 |
| 别 名 | F2RL3; F2R Like Thrombin/Trypsin Receptor 3; Coagulation Factor II(Thrombin) Receptor-Like 3; Thrombin Receptor-Like 3; PAR-4; Coagulation Factor II Receptor-Like 3; Proteinase-Activated Receptor-4; Proteinase-Activated Receptor 4; Protease-Activated Receptor-4; PAR4_HUMAN; |
| 研究领域 | 心血管 细胞生物 信号转导 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 41 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Proteinase-activated receptor 4: 191-385/385 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin. [provided by RefSeq, Jul 2008] Function: Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. Post-translational modifications: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q96RI0 Gene ID: 9002 Database links: Entrez Gene: 9002 Human Omim: 602779 Human SwissProt: Q96RI0 Human Unigene: 137574 Human
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| 产品图片 |
Sample:
Lane 1: Mouse Lung tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Lung tissue lysates
Lane 4: Rat Thyroid gland tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Human K562 cell lysates
Lane 7: Human THP-1 cell lysates
Lane 8: Human Hela cell lysates
Lane 9: Human Huvec cell lysates
Primary: Anti-Proteinase-activated receptor 4/PAR4 (bs-9511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41/38 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
