| 产品编号 | bs-8472R |
| 英文名称 | FBXO11 Rabbit pAb |
| 中文名称 | F-box蛋白相关蛋白11抗体 |
| 别 名 | F box protein 11; F-box only protein 11; FBX11; FBX11_HUMAN; Fbxo11; Fbxo 11; Fbxo-11; PRMT9; VIT-1; VIT1; Vitiligo associated protein VIT 1; Vitiligo-associated protein 1. |
| 研究领域 | 细胞生物 免疫学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Sheep,Cow,Chicken,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 104 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FBXO11: 65-160/927 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the F-box protein familywhich is characterized by an approximately 40 amino acid motif, theF-box. The F-box proteins constitute one of the four subunits ofubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),which function in phosphorylation-dependent ubiquitination. TheF-box proteins are divided into 3 classes: Fbws containing WD-40domains, Fbls containing leucine-rich repeats, and Fbxs containingeither different protein-protein interaction modules or norecognizable motifs. The protein encoded by this gene belongs tothe Fbxs class. It can function as an arginine methyltransferasethat symmetrically dimethylates arginine residues, and it acts asan adaptor protein to mediate the neddylation of p53, which leadsto the suppression of p53 function. This gene is known to bedown-regulated in melanocytes from patients with vitiligo, a skindisorder that results in depigmentation. Polymorphisms in this geneare associated with chronic otitis media with effusion andrecurrent otitis media (COME/ROM), a hearing loss disorder, and theknockout of the homologous mouse gene results in the deaf mousemutant Jeff (Jf), a single gene model of otitis media.Alternatively spliced transcript variants encoding distinctisoforms have been identified for this gene. [provided by RefSeq,Jun 2010]. Function: Substrate recognition component of the a(SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex whichmediates the ubiquitination and subsequent proteasomal degradationof target proteins. Probably recognizes and binds to phosphorylatedtarget proteins. Binds to and neddylates phosphorylated p53/TP53,inhibiting its transcriptional activity. SCF(FBXO11) does not seemto direct ubiquitination of TP53. Subunit: Component of the probable SCF(FBXWO11) complex consisting of CUL1, RBX1, SKP1 and FBXO11. Interacts with TP53. Subcellular Location: Nucleus Tissue Specificity: Isoform 5 is expressed in keratinocytes, fibroblasts and melanocytes. Similarity: Contains 1 F-box domain. Contains 19 PbH1 repeats. Contains 1 UBR-type zinc finger. SWISS: Q86XK2 Gene ID: 80204 Database links: Entrez Gene: 80204 Human Entrez Gene: 225055 Mouse Omim: 607871 Human SwissProt: Q86XK2 Human SwissProt: Q7TPD1 Mouse Unigene: 352677 Human Unigene: 386857 Mouse Unigene: 36585 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
