产品编号 | bs-8202R |
英文名称 | BRN4 |
中文名称 | 脑转录因子4蛋白抗体 |
别 名 | class 3; transcription factor 4; Brain specific homeobox POU domain protein 4; Brain-4; Brain-specific homeobox/POU domain protein 4; BRAIN4; Brn-4; BRN4; DFN3; Oct-9; Octamer-binding protein 9; Octamer-binding transcription factor 9; OTF-9; OTF9; PO3F4_HUMAN; POU domain; POU domain class 3 transcription factor 4; POU3F4. |
研究领域 | 细胞生物 免疫学 神经生物学 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat (predicted: Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1μg /Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 39kDa |
细胞定位 | 细胞核 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BRN4: 211-320/361 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) . A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Function: Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Subcellular Location: Nucleus. Tissue Specificity: Brain specific. DISEASE: Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Similarity: Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: P49335 Gene ID: 5456 Database links: Entrez Gene: 5456 Human Entrez Gene: 18994 Mouse Omim: 300039 Human SwissProt: P49335 Human SwissProt: P62515 Mouse Unigene: 2229 Human Unigene: 405149 Mouse Unigene: 56946 Mouse Unigene: 33030 Rat |
产品图片 |
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-BRN4 antibody(bs-8202R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |