| 产品编号 | bs-8329R |
| 英文名称 | SRXN1 Rabbit pAb |
| 中文名称 | 抗氧化蛋白1抗体 |
| 别 名 | C20orf139; Npn3; SRX; SRX1; 1700127B04Rik; TX01; Ab2-390; SRXN1_HUMAN; SRXN1; 1.8.98.2; SRXN1_MOUSE; Neoplastic progression protein 3; |
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Specific References (3) | bs-8329R has been referenced in 3 publications.
[IF=6.543] Lu Yinzhong. et al. Bilirubin Oxidation End Products (BOXes) Induce Neuronal Oxidative Stress Involving the Nrf2 Pathway. Oxid Med Cell Longev. 2021;2021:8869908 WB ; Mouse.
[IF=2.91] Zhang et al. Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways. (2016) Biosci.Rep. 36 WB ; Rat.
[IF=1.88] Zhou, Yunchuan, et al. "Sulfiredoxin-1 exerts anti-apoptotic and neuroprotective effects against oxidative stress-induced injury in rat cortical astrocytes following exposure to oxygen-glucose deprivation and hydrogen peroxide."International Journal of Molecular Medicine (2015). WB ; Rat.
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 15 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRXN1: 45-137/137 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sulfiredoxin, also designated Sulfiredoxin-1 and chromosome 20 open reading frame 139 (C20orf139), is a cytoplasmic antioxidant protein involved in signaling through catalytic reduction of oxidative modifications. It regulates peroxiredoxins (PRXs), a family of proteins that reduce hydroperoxides, by reducing the conserved cysteine from sulfinic to sulfenic acid. This impacts the role of PRX in the reduction of other downstream transcription factors and kinase signaling pathways. The Sulfiredoxin protein specifically acts on the PRDX1, PRDX2, PRDX3 and PRDX4 peroxiredoxins, but not on PRDX5 or PRDX6. Sulfiredoxin acts as a phosphotransferase and an athioltransferase and is widely expressed, with highest levels detected in lung, spleen, kidney and thymus tissues. Function: SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4. It does not act on PRDX5 or PRDX6. SRXN1 may catalyze the reduction in a multi-step process by acting both as a specific phosphotransferase and a thioltransferase. Subcellular Location: Cytoplasmic Tissue Specificity: Widely expressed with highest levels in kidney, lung, spleen and thymus. Similarity: Belongs to the sulfiredoxin family. SWISS: Q9BYN0 Gene ID: 140809 Database links: Entrez Gene: 140809 Human Entrez Gene: 76650 Mouse SwissProt: Q9BYN0 Human SwissProt: Q9D975 Mouse |
| 产品图片 |
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-SRXN1 (bs-8329R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Lane 4: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-SRXN1 (bs-8329R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 15 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (SRXN1) Polyclonal/MonoclonalAntibody, Unconjugated (bs-8329R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
