| 产品编号 | bs-8334R |
| 英文名称 | Rabbit Anti-NIMP/RTN4IP1 antibody |
| 中文名称 | NOGO相互作用线粒体蛋白1抗体 |
| 别 名 | mitochondrial; NIMP; NOGO interacting mitochondrial protein; NOGO-interacting mitochondrial protein; Reticulon 4 interacting protein 1; Reticulon 4 interacting protein 1, mitochondrial; Reticulon-4-interacting protein 1; RT4I1_HUMAN; Rtn4ip1; NIMP/RTN4IP1. |
| 研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NIMP/RTN4IP1: 111-210/396 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Appears to be a potent inhibitor of regeneration following spinal cord injury.Nogo is an oligodendrocyte-specific member of the Reticulon family and is a component of CNS white matter that inhibits axon outgrowth, induces collapse of growth cones of chick dorsal root ganglion cells, and inhibits the spreading of 3T3 fibroblasts. Nogo is expressed by oligodendrocytes but not by Schwann cells, and associates primarily with the endoplasmic reticulum. Nogo exists in three different splice forms, Nogo-A, -B and -C. NIMP (NOGO-interacting mitochondrial protein), also known as RTN4IP1 (Reticulon-4-interacting protein 1), is a 396 amino acid mitochondrial protein that contains a C-terminal oxidoreductaselike domain and numerous sites for phosphorylation. NIMP is expressed in mitochondrial-rich tissue such as kidney, heart, skeletal muscle and specific regions within the nervous system. Through interaction with Nogo, it is likely that NIMP plays a role in Nogo-induced inhibition of neurite growth. There are three isoforms of NIMP that are produced as a result of alternative splicing events. Function: Appears to be a potent inhibitor of regeneration following spinal cord injury. Subunit: Interacts with RTN4, UQCRC1 and UQCRC2 (By similarity). Subcellular Location: Mitochondrion. Tissue Specificity: Widely expressed in mitochondria-enriched tissues. Found in heart, muscle, kidney, liver, brain and placenta. Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily. SWISS: Q8WWV3 Gene ID: 84816 Database links: Entrez Gene: 84816 Human Entrez Gene: 170728 Mouse Omim: 610502 Human SwissProt: Q8WWV3 Human SwissProt: Q924D0 Mouse Unigene: 155839 Human Unigene: 390253 Mouse Unigene: 220185 Rat |
| 产品图片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti- NIMP/RTN4IP1 (bs-8334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 40 kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- NIMP/RTN4IP1 (bs-8334R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kD
Observed band size: 39 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
