| 产品编号 | bs-8301R |
| 英文名称 | NDUFA9 Rabbit pAb |
| 中文名称 | NDUFA9蛋白抗体 |
| 别 名 | CC6; CI-39k; CI39k; COQ11; MC1DN26; NDUFS2L; SDR22E1; 1010001N11Rik; NDUA9_BOVIN; NDUFA9; Complex I-39kD (CI-39kD); NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUA9_HUMAN; NDUA9_MOUSE; NDUA9_RAT; Sperm flagella protein 3; |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=2ug/test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDUFA9: 131-230/377 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Subunit: Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction. Subcellular Location: Mitochondrion matrix. Similarity: Belongs to the complex I NDUFA9 subunit family. SWISS: Q16795 Gene ID: 4704 Database links: Entrez Gene: 4704 Human Entrez Gene: 66108 Mouse Omim: 603834 Human SwissProt: Q16795 Human SwissProt: Q9DC69 Mouse Unigene: 75227 Human Unigene: 29939 Mouse Unigene: 107963 Rat |
| 产品图片 |
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-NDUFA9 (bs-8301R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-NDUFA9/AF647 Conjugated antibody (bs-8301R-AF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
