| 产品编号 | bs-8066R |
| 英文名称 | SRRM1 |
| 中文名称 | 丝氨酸/精氨酸相关核基质蛋白1抗体 |
| 别 名 | Ser/Arg related nuclear matrix protein; Ser/Arg-related nuclear matrix protein; Serine/arginine repetitive matrix protein 1; SR related nuclear matrix protein of 160 kDa; SR-related nuclear matrix protein of 160 kDa; SRm160; Srrm1; SRRM1_HUMAN. |
| 研究领域 | 细胞生物 染色质和核信号 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Rabbit,Cow,Pig,Dog,Chicken,Rat,Mouse) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 102kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SRRM1: 21-120/904 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. Function: Part of pre- and post-splicing multiprotein mRNPcomplexes. Involved in numerous pre-mRNA processing events.Promotes constitutive and exonic splicing enhancer (ESE)-dependentsplicing activation by bridging together sequence-specific (SRfamily proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP(SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA3'-end cleavage independently of the formation of an exon junctioncomplex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream ofexon-exon junctions. Binds RNA and DNA with low sequencespecificity and has similar preference for either double- orsingle-stranded nucleic acid substrates. Subunit: Identified in the spliceosome C complex. Found in apre-mRNA splicing complex with SFRS4, SFRS5, SNRP70, SNRPA1, SRRM1and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE)complex with SNRP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Found in amRNA splicing-dependent exon junction complex (EJC) with DEK,PRPF8, NCBP1, RBM8A, RNPS1, SRRM1 and ALYREF/THOC4. Interacts withBAT1, CPSF1, RBM8A, RNPS1, and ALYREF/THOC4. Seems to be a compoundof RNA export complexes that are released from speckles in aATP-dependent manner. Subcellular Location: Nucleus matrix. Nucleus speckle. Similarity: Belongs to the splicing factor SR family. Contains 1 PWI domain. SWISS: Q8IYB3 Gene ID: 10250 Database links: Entrez Gene: 10250 Human Entrez Gene: 51796 Mouse Entrez Gene: 100173713 Orangutan Omim: 605975 Human SwissProt: Q8IYB3 Human SwissProt: Q52KI8 Mouse SwissProt: Q5R5Q2 Orangutan Unigene: 18192 Human |
| 产品图片 |
Sample:
Lane 1: Human Jurkat cell lysates Lane 2: Human HeLa cell lysates Lane 3: Human K562 cell lysates Lane 4: Human HL-60 cell lysates Primary: Anti-SRRM1 (bs-8066R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kDa Observed band size: 120 kDa |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
