| 产品编号 | bs-7960R |
| 英文名称 | AMN1 Rabbit pAb |
| 中文名称 | 细胞核重定位及纺锤体检查点蛋白AMN1抗体 |
| 别 名 | AMN1; AMN1_HUMAN; Antagonist of mitotic exit network 1 homolog; Protein AMN1 homolog. |
| 研究领域 | 细胞生物 免疫学 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Cow,Chicken,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 28 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human AMN1: 1-100/258 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
AMN1 is a 258 amino acid protein belonging to the AMN1 family. Encoded by a gene that maps to human chromosome 12p11.21, AMN1 contains twelve degenerate leucine-rich repeat (LRR) motifs and modulates Ste12 binding to promoters of multiple genes. As a daughter cell-specific protein, AMN1 inhibits ELL function to deactivate the mitotic exit state and reset the cell cycle in G1. AMN1 does not halt mitotic exit, but turns it off afterward. Induced by ELL after exit activation, AMN1 disrupts TEM1-Cdc15 interaction by competing with Cdc15 for TEM1 binding and by facilitating inactivation of Cdc14. Defects in AMN1 can result in disruption of both the spindle assembly and nuclear orientation checkpoints. AMN1 may also mediate gene activity and phenotypic diversity. Similarity: Belongs to the AMN1 family. SWISS: Q8IY45 Gene ID: 196394 Database links: Entrez Gene: 196394 Human SwissProt: Q8IY45 Human Unigene: 585084 Human Unigene: 591146 Human Unigene: 104358 Rat |
| 产品图片 |
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(bs-7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMN1 Polyclonal Antibody, Unconjugated(bs-7960R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
