| 产品编号 | bs-7894R |
| 英文名称 | Rabbit Anti-CDCA3 antibody |
| 中文名称 | 细胞分裂周期相关蛋白3抗体 |
| 别 名 | CDCA 3; Cell division cycle associated 3; Cell division cycle associated protein 3; Gene rich cluster C8; Gene rich cluster protein C8; GRCC 8; GRCC8; MGC2577; TOME 1; TOME1; Trigger of mitotic entry 1; C8 antibody; Trigger of mitotic entry protein 1; CDCA3_HUMAN . |
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Specific References (1) | bs-7894R has been referenced in 1 publications.
[IF=3.58] Wang, Qi, et al. "Methamphetamine induces hepatotoxicity via inhibiting cell division, arresting cell cycle and activating apoptosis: In vivo and in vitro studies." Food and Chemical Toxicology (2017). WB ; Rat.
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| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 29kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDCA3: 201-268/268 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase. Function: F-box-like protein which is required for entry into mitosis. Acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase (By similarity). Subunit: Protein modification; protein ubiquitination. [SUBUNIT] Interacts with SKP1. Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity). Subcellular Location: Cytoplasm, cytosol (By similarity). Post-translational modifications: Ubiquitinated and degraded by the APC/C-Cdh1 complex (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. SWISS: Q99618 Gene ID: 83461 Database links: UniProtKB/Swiss-Prot: Q99618.1 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
