| 产品编号 | bs-6912R |
| 英文名称 | Cyclin M2 Rabbit pAb |
| 中文名称 | 周期素M2抗体 |
| 别 名 | ACDP2; Ancient conserved domain containing protein 2; Ancient conserved domain protein 2; Ancient conserved domain-containing protein 2; CNNM 2; CNNM2; CNNM2_HUMAN; Cyclin M2; Cyclin-M2; Metal transporter CNNM2; OTTHUMP00000020387; OTTHUMP00000020388. |
 |
Specific References (1) | bs-6912R has been referenced in 1 publications.
[IF=4.196] Takashina Y et al. Sodium Citrate Increases Expression and Flux of Mg Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells.(2018) Nutrients. Sep 20 ;10(10) WB ; Rat.
|
| 研究领域 | 细胞生物 免疫学 信号转导 细胞周期蛋白 通道蛋白 细胞膜受体 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Dog) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=3ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 96 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cyclin M2/CNNM2: 51-150/875 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Cyclin M2, also known as CNNM2 or ACDP2 (ancient conserved domain-containing protein 2), is an 875 amino acid multi-pass membrane protein that contains two CBS domains and belongs to the ACDP family. Expressed in a variety of tissues with highest expression in placenta, brain and kidney, cyclin M2 functions as a divalent metal cation transporter that mediates the transport of several different metal cations, including Mg2+, Co2+ and Fe2+. Cyclin M2 exists as multiple alternatively spliced isoforms and, contrary to its name, exhibits no cyclin-like function in vivo. The gene encoding cyclin M2 maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman’s syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria. Function: Divalent metal cation transporter. Mediates transport of divalent metal cations in an order of Mg(2+) > Co(2+) > Mn(2+) > Sr(2+) > Ba(2+) > Cu(2+) > Fe(2+ Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed. Expressed at higher level in brain, kidney and placenta, while it is weakly expressed in skeletal muscle. In the kidney, it is expressed in the distal convoluted tubule and the thick ascending limb of Henle loop. DISEASE: Defects in CNNM2 are the cause of hypomagnesemia type 6 (HOMG6) [MIM:613882]. A renal disease characterized by severely lowered serum magnesium levels in the absence of other electrolyte disturbances. Affected individuals show an inappropriately normal urinary magnesium excretion, demonstrating a defect in tubular reabsorption. Age of clinical onset is highly variable and some affected individuals are asymptomatic. Similarity: Belongs to the ACDP family. Contains 2 CBS domains. Contains 1 DUF21 domain. SWISS: Q9H8M5 Gene ID: 54805 Database links: Entrez Gene: 54805 Human Entrez Gene: 94219 Mouse Omim: 607803 Human SwissProt: Q9H8M5 Human SwissProt: Q3TWN3 Mouse Unigene: 643509 Human Unigene: 657970 Human Unigene: 306903 Mouse Unigene: 205139 Rat |
| 产品图片 |
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-Cyclin M2 (bs-6912R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 96 kD
Observed band size: 96 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Cyclin M2 antibody (bs-6912R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protoco
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
