| 产品编号 | bs-7878R |
| 英文名称 | STAM Rabbit pAb |
| 中文名称 | 信号转导衔接蛋白1抗体 |
| 别 名 | Signal transducing adapter molecule 1; Signal transducing adapter molecule 2; Signal transducing adaptor molecule(SH3 domain and ITAM motif) 1; Signal transducing adaptor molecule; STAM 1; STAM-2; STAM1; Stam2; STAM2_HUMAN; DKFZp686J2352; Hrs-binding protein; HSE1 homolog; OTTHUMP00000019237. |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human STAM: 101-200/540 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. Function: Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity). Subunit: Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Probably part of a complex at least composed of HSG, STAM and EPS15. Interacts with STAMBP/AMSH. Interacts with PDGFRB. Subcellular Location: Cytoplasm. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF. Similarity: Belongs to the STAM family. Contains 1 ITAM domain. Contains 1 SH3 domain. Contains 1 UIM (ubiquitin-interacting motif) repeat. Contains 1 VHS domain. SWISS: Q92783 Gene ID: 8027 Database links: Entrez Gene: 10254 Human Entrez Gene: 8027 Human Entrez Gene: 20844 Mouse Entrez Gene: 56324 Mouse Omim: 601899 Human SwissProt: O75886 Human SwissProt: Q92783 Human SwissProt: O88811 Mouse SwissProt: P70297 Mouse Unigene: 17200 Human Unigene: 263639 Mouse Unigene: 161793 Rat |
| 产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-STAM Polyclonal Antibody, Unconjugated(bs-7878R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
