| 产品编号 | bs-7874R |
| 英文名称 | SART1 |
| 中文名称 | T淋巴细胞识别的鳞状细胞癌抗原抗体 |
| 别 名 | Ara 1; ARA1; HOMS 1; HOMS1; hSART 1; hSART1; hSnu 66; hSnu66; IgE autoantigen; MGC2038; SART 1; SART1 259; SART1(259) protein; SART1(800) protein; SART1259; Snu 66; Snu66; Squamous cell carcinoma antigen recognised by T cells; Squamous cell carcinoma antigen recognized by T cells 1; U4/U6.U5 tri snRNP associated 110 kDa protein; U4/U6.U5 tri snRNP associated protein; SNUT1_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 细胞周期蛋白 细胞分化 t-淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Pig,Cow,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 90kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SART1: 701-800/800 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway. Function: May play a role in mRNA splicing. May also bind to DNA. Subunit: Identified in the spliceosome C complex. Part of a tri-snRNP complex. Subcellular Location: Nucleus. Note=Found in the nucleus of mitogen-activated peripheral blood mononuclear cells (PBMCs), tumor cells, or normal cell lines, but not in normal tissues except testis and fetal liver or in unstimulated PBMCs, suggesting preferential expression in proliferating cells. Tissue Specificity: Ubiquitously expressed. Post-translational modifications: Sumoylated with SUMO2. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the SNU66/SART1 family. SWISS: O43290 Gene ID: 9092 |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
