| 产品编号 | bs-6909R |
| 英文名称 | Rabbit Anti-BMP9 antibody |
| 中文名称 | 骨形态发生蛋白9抗体 |
| 别 名 | BMP 9; BMP-9; Bone morphogenetic protein 9; GDF 2; GDF2; GDF-2; growth differentiation factor 2; GDF2_HUMAN. |
| 研究领域 | 细胞生物 免疫学 信号转导 生长因子和激素 细胞周期蛋白 转录调节因子 细胞分化 细胞外基质 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Rabbit) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12/47 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BMP9: 351-429/429 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein plays a role in the adult liver and in differentiation of cholinergic central nervous system neurons. [provided by RefSeq, Jul 2008]. Function: Could be involved in bone formation. Subunit: Homodimer; disulfide-linked. Subcellular Location: Secreted. Similarity: Belongs to the TGF-beta family. SWISS: Q9UK05 Gene ID: 2658 Database links: Entrez Gene: 2658 Human Entrez Gene: 12165 Mouse Omim: 605120 Human SwissProt: Q9UK05 Human SwissProt: Q9WV56 Mouse Unigene: 218418 Rat |
| 产品图片 |
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-BMP9 (bs-6909R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12/47 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP9) Polyclonal Antibody, Unconjugated (bs-6909R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
