| 产品编号 | bs-7848R |
| 英文名称 | SKA3 Rabbit pAb |
| 中文名称 | 纺锤体和着丝粒相关蛋白3抗体 |
| 别 名 | C13orf3; RAMA1; SKA3; SKA3_HUMAN; Spindle and kinetochore associated complex subunit 3; Spindle and kinetochore-associated protein 3. |
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Specific References (2) | bs-7848R has been referenced in 2 publications.
[IF=5.959] Hou Y et al. SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma. Cell Death Dis. 2019 Dec 5;10(12):929. WB&IHC-P ; Human.
[IF=2.538] Haoyue Xu. et al. SKA3 promotes cell growth via the PI3K/AKT/GSK3β and PI3K/AKT/FOXO1 pathways and is a potential prognostic biomarker for oral squamous cell carcinoma. OR SURG OR MED OR PA. 2022 Jun;: WB, IHC ; Human, Mouse.
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| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Sheep,Cow,Chicken,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 46 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SKA3: 201-300/412 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. Function: Component of the SKA1 complex, a microtubule-bindingsubcomplex of the outer kinetochore that is essential for properchromosome segregation. The SKA1 complex is a direct component ofthe kinetochore-microtubule interface and directly associates withmicrotubules as oligomeric assemblies. The complex facilitates theprocessive movement of microspheres along a microtubule in adepolymerization-coupled manner. In the complex, it mediates themicrotubule-stimulated oligomerization. Subunit: Component of the SKA1 complex, composed of SKA1, SKA2 andSKA3. The core SKA1 complex is composed of 2 SKA1-SKA2heterodimers, each heterodimer interacting with a molecule of theSKA3 homodimer. The core SKA1 complex associates with microtubulesand forms oligomeric assemblies. Interacts directly with SKA1. Subcellular Location: Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner. Similarity: Belongs to the SKA3 family. SWISS: Q8IX90 Gene ID: 221150 Database links: Entrez Gene: 221150 Human Entrez Gene: 219114 Mouse SwissProt: Q8IX90 Human SwissProt: Q8C263 Mouse Unigene: 88523 Human Unigene: 30173 Mouse Unigene: 218204 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SKA3) Polyclonal Antibody, Unconjugated (bs-7848R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
