| 产品编号 | bs-7847R |
| 英文名称 | Rabbit Anti-SKA2 antibody |
| 中文名称 | 纺锤体和着丝粒相关蛋白2抗体 |
| 别 名 | FAM33A; Family with sequence similarity 33, member A; FLJ12758; MGC110975; Protein FAM33A; SKA 2; SKA2; SKA2_HUMAN; Spindle and kinetochore associated complex subunit 2; Spindle and kinetochore associated protein 2; Spindle and kinetochore-associated protein 2; Spindle and KT (kinetochore) associated 2; Spindle and KT associated 2. |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SKA2: 2-88/121 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ska2 (spindle and kinetochore associated complex subunit 2), also known as FAM33A, is a 121 amino acid component of the Ska1 complex, a microtubule-binding subcomplex of the outer kinetochore that is critical for proper chromosome segregation. The Ska1 complex is a component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. Localized to the outer kinetochore and spindle microtubules during cell proliferation, Ska2 is essential for spindle checkpoint silencing and exit from mitosis. Downregulation of Ska2 leads to delayed recruitment of MAD2, a component of the mitotic spindle checkpoint, to several kinetochores resulting in occasional loss of individual chromosomes from the metaphase plate. Ska2 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. Function: Component of the SKA1 complex, a microtubule-bindingsubcomplex of the outer kinetochore that is essential for properchromosome segregation. Required for timely anaphase onset duringmitosis, when chromosomes undergo bipolar attachment on spindlemicrotubules leading to silencing of the spindle checkpoint. TheSKA1 complex is a direct component of the kinetochore-microtubuleinterface and directly associates with microtubules as oligomericassemblies. The complex facilitates the processive movement ofmicrospheres along a microtubule in a depolymerization-coupledmanner. In the complex, it is required for SKA1 localization. Subunit: Component of the SKA1 complex, composed of SKA1, SKA2 andSKA3. Forms a heterodimer with SKA1; the heterodimer interactingwith SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2heterodimers, each heterodimer interacting with a molecule of theSKA3 homodimer. The core SKA1 complex associates with microtubulesand forms oligomeric assemblies. Interacts directly with SKA1.Binds directly to microtubules; but with a much lower affinity thanSKA1. May interact with NR3C1; the relevance of such interactionremains unclear in vivo. Subcellular Location: Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner. Localizes to both the mitotic spindle andkinetochore-associated proteins. Similarity: Belongs to the SKA2 family. SWISS: Q8WVK7 Gene ID: 348235 Database links: Entrez Gene: 348235 Human Entrez Gene: 66140 Mouse SwissProt: Q8WVK7 Human SwissProt: Q9CR46 Mouse Unigene: 463607 Human Unigene: 45008 Mouse Unigene: 203397 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
