| 产品编号 | bs-7844R |
| 英文名称 | Rabbit Anti-NIN antibody |
| 中文名称 | GSK3B相互作用蛋白抗体 |
| 别 名 | Centrosome Marker; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; Centrosome Marker; Glycogen synthase kinase 3 beta interacting protein; GSK3B interacting protein; h Glycogen synthase kinase 3 beta-interacting protein; hNinein; KIAA1565; NIN protein; NIN_HUMAN; ninein (GSK3B interacting protein); Ninein; Ninein centrosomal protein; SCKL7. |
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Specific References (1) | bs-7844R has been referenced in 1 publications.
[IF=2.1] Nozawa‐Inoue, Kayoko, et al. "Contribution of synovial lining cells to synovial vascularization of the rat temporomandibular joint." Journal of Anatomy (2016). IHC-F ; Rat.
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| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 243kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ninein: 133-230/2090 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Subunit: Homooligomer. Interacts with GSK3B (GSK3-beta) via its C-terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3-beta. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Tissue Specificity: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. Post-translational modifications: Phosphorylated by Aurora A (AIK) and PKA kinases but not CK2 or Aurora B (AIM). Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Contains 5 EF-hand domains. SWISS: Q8N4C6 Gene ID: 51199 Database links: Entrez Gene: 51199 Human Entrez Gene: 18080 Mouse Omim: 608684 Human SwissProt: Q8N4C6 Human SwissProt: Q61043 Mouse Unigene: 310429 Human Unigene: 569387 Human Unigene: 244047 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
