| 产品编号 | bs-7828R |
| 英文名称 | Rabbit Anti-RINT1 antibody |
| 中文名称 | 胞膜间内质网调节蛋白1抗体 |
| 别 名 | HsRINT 1; HsRINT1; RAD50 interacting protein 1; RINT 1; RINT1_HUMAN. |
| 研究领域 | 细胞生物 信号转导 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 91kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RINT1: 201-300/792 |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
RINT1 is involved in the regulation of membrane traffic between the golgi and the endoplasmic reticulum. It may play a role in cell cycle checkpoint control and is essential for telomere length control. Function: Involved in regulation of membrane traffic between theGolgi and the endoplasmic reticulum. May play a role in cell cyclecheckpoint control. Essential for telomere length control. Subunit: Associated with a SNARE complex consisting of STX18,USE1L, BNIP1/SEC20L, and SEC22B. Interacts directly withBNIP1/SEC20L and ZW10. Interacts with RAD50 during late S and G2/Mphases. Interacts with RBL2, preferentially with the active,hypophosphorylated form. Subcellular Location: Cytoplasm. Endoplasmic reticulum membrane;Peripheral membrane protein. Similarity: Belongs to the RINT1 family. Contains 1 RINT1/TIP20 domain. SWISS: Q6NUQ1 Gene ID: 60561 Database links: UniProtKB/Swiss-Prot: Q6NUQ1.1 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RCL) Polyclonal Antibody, Unconjugated (bs-7828R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
