| 产品编号 | bs-7827R |
| 英文名称 | RCL Rabbit pAb |
| 中文名称 | c-Myc应答蛋白抗体 |
| 别 名 | c Myc responsive; c Myc responsive protein Rcl; C6orf108; Chromosome 6 open reading frame 108; Putative c Myc responsive; RCL_HUMAN. |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 19 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RCL/c Myc responsive: 101-174/174 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene was identified on the basis of its stimulationby c-Myc protein. The latter is a transcription factor thatparticipates in the regulation of cell proliferation,differentiation, and apoptosis. The exact function of this gene isnot known but studies in rat suggest a role in cellularproliferation and c-Myc-mediated transformation. Two alternativetranscripts encoding different proteins have been described.[provided by RefSeq, Jul 2008]. Function: Catalyzes the cleavage of the N-glycosidic bond ofdeoxyribonucleoside 5'-monophosphates to yield deoxyribose5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside5'-monophosphates containing purine bases are preferred to thosecontaining pyrimidine bases (By similarity). Subunit: Monomer and homodimer (By similarity). Subcellular Location: Nucleus (By similarity). Cytoplasm. Tissue Specificity: Expressed at low levels in brain, colon, lung,peripheral blood leukocytes, placenta, small intestine, and thymus.Expressed at high levels in heart, kidney, liver, skeletal muscleand spleen. Overexpressed in a significant proportion of breastcancers. Similarity: Belongs to the deoxyribonucleoside 5'-monophosphateN-glycosidase family SWISS: O43598 Gene ID: 10591 Database links: Entrez Gene: 10591 Human SwissProt: O43598 Human Unigene: 109752 Human |
| 产品图片 |
Sample:
Kidney (Rat) Lysate at 40 ug
Liver (Rat) Lysate at 40 ug
Primary: Anti-RCL (bs-7827R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 19 kD
Observed band size: 19 kD
We are unsure as to the identity of these extra bands
May be formed of homo-dimer
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RCL/c Myc responsive Polyclonal Antibody, Unconjugated(bs-7827R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
