| 产品编号 | bs-7773R |
| 英文名称 | SPECC1L Rabbit pAb |
| 中文名称 | 细胞质和纺锤体机化蛋白A抗体 |
| 别 名 | Cytokinesis and spindle organization A; Cytospin-A; CYTSA; CYTSA_HUMAN; Renal carcinoma antigen NY-REN-22; SPECC1-like protein; specc1l; Sperm antigen with calponin homology and coiled-coil domains 1-like. |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 125 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPECC1L: 161-260/1117 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013] Function: Involved in cytokinesis and spindle organization. May play a role in actin cytoskeleton organization and microtubule stabilization and hence required for proper cell adhesion and migration. Subunit: May interact with both microtubules and actin cytoskeleton. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cell junction, gap junction. Note=Colocalizes with acetylated alpha-tubulin, gamma-tubulin and F-actin. Also observed in a ring around gamma-tubulin containing centrioles possibly in the microtubule organizing center. DISEASE: Defects in SPECC1L are the cause of facial clefting oblique type 1 (OBLFC1) [MIM:600251]. A rare form of facial clefting. A facial cleft is any of the fissures between the embryonic prominences that normally unite to form the face. Similarity: Belongs to the cytospin-A family. Contains 1 CH (calponin-homology) domain. SWISS: Q69YQ0 Gene ID: 23384 Database links: Entrez Gene: 23384 Human Entrez Gene: 74392 Mouse SwissProt: Q69YQ0 Human SwissProt: Q2KN98 Mouse Unigene: 474384 Human |
| 产品图片 |
Sample:
HL60(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
HL60 (Human) Cell Lysate at 30 ug
Primary: Anti- SPECC1L (bs-7773R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 125 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
